Sanitary Environmental Engineering Division · University of Salerno
Patented technology · SEED, University of Salerno

The membrane is alive. Clean, safe water, almost no fouling.

Living Membrane® turns the biofilm into the filter. Two coarse meshes encapsulate a living biological layer that builds itself during treatment, producing reuse-grade water at low pressure, low energy and with almost no membrane cleaning.

100 L pilot · real urban wastewater 0 external membrane cleaning UniSA SEED patented technology
Living Membrane · cross-section Anode Cathode
cake
encapsulated biofilm
Influent · raw wastewater Permeate · reuse-grade
Electro mode
Developed & scaled within
SPORE-MED PRIMA ASIS Salernitana University of Salerno
02
Evidence

Proven on real urban wastewater.

A 100-litre pilot ran continuously on raw municipal wastewater at the Battipaglia treatment plant. Through wide swings in the incoming load, the Living Membrane held a stable filter and barely fouled, retaining microplastics and helping to control contaminants of emerging concern such as antibiotics and pharmaceuticals.

0%
Polyethylene microplastics retained
0%
Silicone (PDMS) microplastics retained
0%
COD (organic load) removal
0%
Ammonia (NH₄-N) removal
0NTU
Permeate turbidity, stable filter
0cycles
External membrane cleaning, negligible fouling

Start-up performance of the 100-litre pilot-scale Living Membrane bioreactor · Battipaglia WWTP, Campania, Italy · 2026.

03
Technology

Fouling, reimagined as filtration.

In a conventional membrane bioreactor, the biofilm that builds on the membrane is the enemy: it clogs, it raises pressure, it demands chemicals and downtime. Living Membrane inverts the problem: it cultivates that layer on purpose and uses it as the active filter.

i

It builds itself

No fine manufactured membrane to buy, foul or replace. A living cake and an encapsulated biofilm self-form on two coarse meshes during normal operation.

ii

The biofilm is encapsulated

Two adjacent coarse mesh sheets trap a biological layer between them. Solids are intercepted in series, first an external cake, then the encapsulated biofilm, giving consistently low permeate turbidity.

iii

Low pressure, low energy

The system runs at very low transmembrane pressure with an extremely low fouling rate, enabling continuous operation with no chemical cleaning.

iv

Electro-enhanced option

In the electro-LMBR variant, two electrodes in the tank set up a mild electric field that drives charged foulants away from the membrane, protecting the filter and sharpening removal. Switch on Electro mode on the diagram above to watch the foulants migrate.

04
Maturity

From bench to basin.

More than a decade of research at the SEED laboratory, advanced from synthetic solutions to a working pilot on real wastewater under the EU-funded SPORE-MED project.

TRL 3–4 · Laboratory

The principle

Living, self-forming dynamic bio-membranes validated on synthetic urban and textile wastewater: high removals, low fouling, and the core technology patented.

TRL 5–6 · Pilot · now

Real wastewater

100 L pilot at Battipaglia WWTP treats raw municipal influent within SPORE-MED. Stable filter, microplastic retention, no external cleaning.

TRL 7+ · Scale-up

Demonstration & uptake

Roadmap to demonstration-scale modules and full-scale retrofits for utilities upgrading to meet the revised EU Urban Wastewater Treatment Directive.

The Battipaglia pilot

Real plant. Real water. Real conditions.

The pilot is installed inside an operating urban wastewater treatment plant in Battipaglia, Campania, and fed with the effluent of the plant's preliminary treatment stage, the same variable and complex matrix a full-scale works must handle every day.

  • SiteBattipaglia WWTP, Campania · operated by ASIS Salernitana Reti e Impianti S.p.A.
  • FeedRaw municipal wastewater after preliminary treatment
  • Scale100 L bioreactor with a module of electro Living Membrane
  • FundingDesigned and built within SPORE-MED · PRIMA programme, EU (Reference 2322)
  • ResultContinuous operation, stable permeate, no external cleaning
Pilot-scale electro Living Membrane bioreactor schematic Process schematic: urban wastewater feeds a 100 L open-top bioreactor housing an electro Living Membrane module. Two cathodes flank the module and two anodes sit near the reactor walls, all wired to a DC supply. Air diffusers aerate the tank; permeate is drawn through a transmembrane-pressure sensor and pump; a periodic backwash line returns to the module. FIG. 1 · PILOT-SCALE ELECTRO LIVING MEMBRANE BIOREACTOR URBAN WASTEWATER after preliminary treatment FEED PUMP DC SUPPLY + + + ELECTRO LIVING MEMBRANE MODULE AIR SUPPLY 100 L BIOREACTOR open-top reactor PERIODIC BACKWASH TMP PERMEATE PUMP PERMEATE reuse-grade water Process schematic · electro Living Membrane Bioreactor · pilot-plant operated within SPORE-MED project (Reference 2322) part of the PRIMA Programme, supported by the European Union:
05
Applications

Where a living filter changes the economics.

Upgrading urban WWTPs

A low-energy, small-footprint route to advanced treatment for works modernising under the revised EU Urban Wastewater Treatment Directive (2024/3019).

Directive-ready

Microplastics & emerging contaminants

High retention of microplastics on real wastewater, and a barrier for contaminants of emerging concern such as antibiotics and pharmaceuticals.

Emerging contaminants

Water reuse

Reuse-grade, low-turbidity permeate for agricultural irrigation and non-potable reuse in water-stressed Mediterranean regions.

Circular water

Industrial & textile effluent

Demonstrated on textile wastewater with model dyes, with robust treatment for difficult, high-strength industrial streams.

Industrial

Low energy & footprint

A self-forming membrane and the absence of chemical cleaning lower energy use and operating costs, while the patented support module can be built from eco-compatible materials.

Low energy & eco

Resource recovery

Part of a circular-economy treatment train, recovering water and nutrients while keeping plants compact and efficient.

Nutrient & water
06
Research

Peer-reviewed, patent-protected.

Living Membrane is the product of a sustained research line at the Sanitary Environmental Engineering Division (SEED) of the University of Salerno. Its performance and potential are documented in several papers in high-impact scientific journals.

Patented technology
The next generation of Self-forming dynamic membrane bioreactor
Living Membrane is patented technology of the University of Salerno (SEED), integrating biological treatment with dynamic membrane filtration.
Scientific evidence
Published in high-impact journals
Several peer-reviewed studies report the technology's performance and potential for advanced wastewater treatment, microplastic and emerging-contaminant control, and fouling mitigation.
Team
Developed by SEED, University of Salerno
Led by Prof. Vincenzo Naddeo with the SEED group, within the SPORE-MED partnership. Interested in the data or a collaboration? Get in touch.
About · a result of SPORE-MED

Scaled within a Mediterranean mission.

Living Membrane is developed and scaled within SPORE-MED, Sustainable upgraded wastewater treatment plants for resource recovery, water reuse and health surveillance in the Mediterranean region, a three-year project (2024–2027) coordinated by the University of Girona under the PRIMA programme and funded by the European Union (GA 2322). This website is one of the project's dissemination outputs.

Visit the SPORE-MED project site →

Univ. de Girona · LEQUIAUniv. of Salerno · SEEDUniv. of CyprusTechnical Univ. of CreteUniv. of SfaxUM6PUniv. Autònoma de BarcelonaGS InimaAdasa Sistemas

Bring a living filter to your plant.

We work with utilities, industry and researchers on licensing, demonstration and joint development of the Living Membrane technology.